Twig offers a variety of options that you can pass to the Environment, to do this with Twilight, you may hook into the twig:init
event and modify the instance. Here's an example of adding a custom Twig function:
<?php
use Twig\TwigFunction;
use Twig\Environment;
use Twilight\Events;
Events::on( 'twig:init', function( Environment $twig ) {
$twig->addFunction( new TwigFunction( 'do_a_thing', 'do_a_thing' ) );
} );